The world around your pet is teeming with microscopic organisms, some beneficial and others potentially harmful. At Veterinary Diagnostic Centre, our Microbiology department delves into this hidden world, identifying and combating infectious agents that can compromise your pet’s health.
The acid-fast stain is a differential stain used to identify acid-fast and partial acid-fast organisms, mainly the members of the genus Mycobacterium & Nocardia.
To differenciate between Gram positive and Gram negative organism, if present any
Aerobic culture is used for the isolation and identification of most aerobic and facultative anaerobic bacteria. An antimicrobial susceptibility test will be performed when organisms isolated meet microbiologic criteria for clinical significance or unless otherwise requested
This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.
For isolating and identifying most clinically significant anaerobic bacteria.
Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture.
To isolate and identify possible pathogens responsible for mastitis.
To isolate and identify fungal pathogens.
To isolate and identify
To isolate and identify dermatophytes.
This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.
The acid-fast stain is a differential stain used to identify acid-fast and partial acid-fast organisms, mainly the members of the genus Mycobacterium & Nocardia.
Test Turnaround | 1 day |
Specimen Required | Swab, Tissue, Exudate, Prepared air dried slide |
To differenciate between Gram positive and Gram negative organism, if present any
Test Turnaround | 1 day |
Specimen Required | Gram’s stain is used as a differencial stain for isolated bacteria and also can be used for direct smear examination |
Aerobic culture is used for the isolation and identification of most aerobic and facultative anaerobic bacteria. An antimicrobial susceptibility test will be performed when organisms isolated meet microbiologic criteria for clinical significance or unless otherwise requested
Test Turnaround | 2-7 days |
Specimen Required | Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates |
Collection Container | Sterile, leak-proof container or Bacterial transport medium |
Special Instructions | If a specific organism is suspected, please note this on the submission form; this information can assist with proper culture setup. Please note specimens are not routinely pooled for aerobic cultures unless requested. |
This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.
Test Turnaround | 2-7 days |
Specimen Required | Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates |
Collection Container | Sterile, leak-proof container or Bacterial transport medium |
Special Instructions | For urine culture see Urine Culture and Sensitivity test Specimen should be collected to limit bacterial contamination. After collection, specimen should be refrigerated promptly after collection to prevent overgrowth, with the exception of blood, joint and CSF specimen which should be kept at room temperature. Samples are not routinely pooled for aerobic cultures unless requested. |
For isolating and identifying most clinically significant anaerobic bacteria
Test Turnaround | 2-7 days |
Specimen Required | Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates |
Collection Container | Sterile, leak-proof container or Port-a-cul system or other obligate anaerobe transport media (Amies gel swabs are not appropriate) |
Special Instructions | If submitting swabs for culture from specimen that may contain both aerobic and anaerobic growth the swab must be able to maintain both aerobic and anaerobic bacterial viability, such as a Port-a-cul system or other obligate anaerobe transport media. Note, transport systems with Amies gel is for facultative anaerobic bacteria and are not appropriate for obligate anaerobes. For Clostridium perfringens and Clostridioides difficile (formerly Clostridium difficile) please use the Test Search Tool for more specific information related to testing for these organisms. Samples are not routinely pooled for anaerobic cultures unless requested |
Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture
Species | All species |
Test Turnaround | 3-7 days |
Specimen Required | Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture |
Collection Container | Sterile, leak-proof container or Bacterial transport medium |
To isolate and identify microorganisms from blood.
Species | All species |
Specimen Required | Samples should be fresh and collected aseptically |
Test Turnaround | 3-7 days |
Specimen Criteria | Samples should be fresh and collected aseptically |
Ideal Sample Volume | 3ml |
Min Sample Volume | 1ml |
Rejection Criteria | Specimen contaminated by external particles. Sample kept at room temperature for more than 24 hours |
Collection Container | Blood culture vials |
To isolate and identify possible pathogens from milk
Specimen Required | Samples should be fresh and collected aseptically |
Test Turnaround | 3-7 days |
Specimen Criteria | Samples should be fresh and collected aseptically |
Ideal Sample Volume | 3ml |
Min Sample Volume | 1ml |
Rejection Criteria | Specimen contaminated by external particles. Sample kept at room temperature for more than 12 hours |
To isolate and identify possible pathogens responsible for mastitis
Test Turnaround |
2-7 days |
Specimen Required |
Samples should be fresh and collected aseptically
|
Specimen Criteria |
Samples should be fresh and collected aseptically
|
Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture
Species | All species |
Specimen Required | Samples should be fresh and collected aseptically |
Test Turnaround | 3-7 days |
Specimen Criteria | Samples should be fresh and collected aseptically |
Ideal Sample Volume | 3ml |
Min Sample Volume | 1ml |
Rejection Criteria | Specimen contaminated by external particles. Sample kept at room temperature for more than 24 hours |
Collection Container | Blood culture vials |
To isolate and identify Mycobacterium sp. from clinical samples
Species | All species |
Specimen Required | Samples should be fresh and collected aseptically |
Test Turnaround | 15-21 day |
Specimen Criteria | Nasal wash |
Ideal Sample Volume | 3ml |
Min Sample Volume | 2ml |
Rejection Criteria | Specimen contaminated by external particles. Sample kept at room temperature for more than 24 hours |
Collection Container | Blood culture vials |
To isolate and identify fungal pathogens
Species | All species |
Specimen Required | Samples should be fresh and collected aseptically |
Test Turnaround | 7-14 days |
Specimen Criteria | Hair, Scabs, Tissue, Body fluids |
Collection Container | Hair/ Scabs can be enclosed in plastic bag or paper. Body fluids and tissue should be collected in sterile leak proof container. |
To isolate and identify
To isolate and identify dermatophytes
Species | All species |
Test Turnaround | 7-14 days |
Specimen Criteria | Hair, Scabs, Tissue, Body fluids |
Ideal Sample Volume | 2-5g |
Min Sample Volume | 2g |
This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.
Test Turnaround | 2-5 days |
Specimen Required | Urine |