VDC

Lab Tests

Microbiology

The world around your pet is teeming with microscopic organisms, some beneficial and others potentially harmful. At Veterinary Diagnostic Centre, our Microbiology department delves into this hidden world, identifying and combating infectious agents that can compromise your pet’s health.

Acid Fast Stain
Description

The acid-fast stain is a differential stain used to identify acid-fast and partial acid-fast organisms, mainly the members of the genus Mycobacterium & Nocardia.

Gram Stain
Description

To differenciate between Gram positive and Gram negative organism, if present any

Aerobic Culture
Description

Aerobic culture is used for the isolation and identification of most aerobic and facultative anaerobic bacteria. An antimicrobial susceptibility test will be performed when organisms isolated meet microbiologic criteria for clinical significance or unless otherwise requested

Aerobic Culture and Sensitivity Test
Description

This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.

Anaerobic Culture
Description

For isolating and identifying most clinically significant anaerobic bacteria.

Antibiotic Sensitivity Test
Description

Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture.

Milk Culture (Mastitis)
Description

To isolate and identify possible pathogens responsible for mastitis.

Mycology Culture
Description

To isolate and identify fungal pathogens.

Mycoplasma Culture
Description

To isolate and identify

Dermatophyte culture
Description

To isolate and identify dermatophytes.

Urine Culture and Sensitivity Test
Description

This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.

Acid Fast Stain
Description

The acid-fast stain is a differential stain used to identify acid-fast and partial acid-fast organisms, mainly the members of the genus Mycobacterium & Nocardia.

Test Turnaround1 day
Specimen Required
Swab, Tissue, Exudate, Prepared air dried slide
Gram Stain
Description

To differenciate between Gram positive and Gram negative organism, if present any

Test Turnaround1 day
Specimen Required
Gram’s stain is used as a differencial stain for isolated bacteria and also can be used for direct smear examination
Aerobic Culture
Description

Aerobic culture is used for the isolation and identification of most aerobic and facultative anaerobic bacteria. An antimicrobial susceptibility test will be performed when organisms isolated meet microbiologic criteria for clinical significance or unless otherwise requested

Test Turnaround
2-7 days
Specimen Required
Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates
Collection ContainerSterile, leak-proof container or Bacterial transport medium
Special Instructions
If a specific organism is suspected, please note this on the submission form; this information can assist with proper culture setup. Please note specimens are not routinely pooled for aerobic cultures unless requested.
Aerobic Culture and Sensitivity Test
Description

This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.

Test Turnaround
2-7 days
Specimen Required
Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates
Collection ContainerSterile, leak-proof container or Bacterial transport medium
Special Instructions
For urine culture see Urine Culture and Sensitivity test Specimen should be collected to limit bacterial contamination. After collection, specimen should be refrigerated promptly after collection to prevent overgrowth, with the exception of blood, joint and CSF specimen which should be kept at room temperature. Samples are not routinely pooled for aerobic cultures unless requested.
Anaerobic Culture
Description

For isolating and identifying most clinically significant anaerobic bacteria

Test Turnaround
2-7 days
Specimen Required
Fresh tissues, Fluids, Exudates, Aspirates, Pure bacterial isolates
Collection Container
Sterile, leak-proof container or Port-a-cul system or other obligate anaerobe transport media (Amies gel swabs are not appropriate)
Special Instructions
If submitting swabs for culture from specimen that may contain both aerobic and anaerobic growth the swab must be able to maintain both aerobic and anaerobic bacterial viability, such as a Port-a-cul system or other obligate anaerobe transport media. Note, transport systems with Amies gel is for facultative anaerobic bacteria and are not appropriate for obligate anaerobes. For Clostridium perfringens and Clostridioides difficile (formerly Clostridium difficile) please use the Test Search Tool for more specific information related to testing for these organisms. Samples are not routinely pooled for anaerobic cultures unless requested
Antibiotic Sensitivity Test
Description

Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture

Species
All species
Test Turnaround
3-7 days
Specimen Required
Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture
Collection Container
Sterile, leak-proof container or Bacterial transport medium
Blood culture
Description

To isolate and identify microorganisms from blood.

Species
All species
Specimen Required
Samples should be fresh and collected aseptically
Test Turnaround3-7 days
Specimen Criteria
Samples should be fresh and collected aseptically
Ideal Sample Volume3ml
Min Sample Volume
1ml
Rejection CriteriaSpecimen contaminated by external particles. Sample kept at room temperature for more than 24 hours
Collection ContainerBlood culture vials
Milk culture (Bulk tank)
Description

To isolate and identify possible pathogens from milk

Specimen Required
Samples should be fresh and collected aseptically
Test Turnaround
3-7 days
Specimen CriteriaSamples should be fresh and collected aseptically
Ideal Sample Volume
3ml
Min Sample Volume1ml
Rejection Criteria
Specimen contaminated by external particles. Sample kept at room temperature for more than 12 hours
Milk Culture (Mastitis)
Description

To isolate and identify possible pathogens responsible for mastitis

Test Turnaround
2-7 days
Specimen Required
Samples should be fresh and collected aseptically
Specimen Criteria
Samples should be fresh and collected aseptically
Campylobacter culture
Description

Sensitivities of various antibiotics to isolated bacteria is estimated through aerobic culture

Species
All species
Specimen Required
Samples should be fresh and collected aseptically
Test Turnaround3-7 days
Specimen Criteria
Samples should be fresh and collected aseptically
Ideal Sample Volume3ml
Min Sample Volume
1ml
Rejection CriteriaSpecimen contaminated by external particles. Sample kept at room temperature for more than 24 hours
Collection ContainerBlood culture vials
Mycobacterium Culture
Description

To isolate and identify Mycobacterium sp. from clinical samples

Species
All species
Specimen Required
Samples should be fresh and collected aseptically
Test Turnaround
15-21 day
Specimen Criteria
Nasal wash
Ideal Sample Volume3ml
Min Sample Volume
2ml
Rejection CriteriaSpecimen contaminated by external particles. Sample kept at room temperature for more than 24 hours
Collection ContainerBlood culture vials
Mycology Culture
Description

To isolate and identify fungal pathogens

Species
All species
Specimen Required
Samples should be fresh and collected aseptically
Test Turnaround
7-14 days
Specimen Criteria
Hair, Scabs, Tissue, Body fluids
Collection Container
Hair/ Scabs can be enclosed in plastic bag or paper. Body fluids and tissue should be collected in sterile leak proof container.
 
Mycoplasma Culture
Description

To isolate and identify

Dermatophyte culture
Description

To isolate and identify dermatophytes

Species
All species
Test Turnaround
7-14 days
Specimen Criteria
Hair, Scabs, Tissue, Body fluids
Ideal Sample Volume
2-5g
Min Sample Volume
2g
 
Urine Culture and Sensitivity Test
Description

This test is a combination of aerobic culture and antibiotic susceptibility test. If no pathogen is isolated and identified no susceptibility is performed. More than one antibiotic susceptibility will be tested if more than one bacteria is isolated.

Test Turnaround
2-5 days
Specimen Required
Urine